PDA

View Full Version : how to remove the Bio filim in water system



jmtbrahma
09-16-2008, 04:45 PM
Dear All,
How to remove the Biofilm in water system, please suggest.

Regards
jmtbrahma

wito
09-17-2008, 08:35 PM
It suggests passivation. It is possible steam - but requirement of removal of delicate element is from all installation.

jmtbrahma
09-19-2008, 03:32 PM
It suggests passivation. It is possible steam - but requirement of removal of delicate element is from all installation.

what type of passivation.

wito
09-20-2008, 09:14 AM
passivation only for new systems. I had a problem with the extension growth of microorganisms in the WFI, removed by steam to provide water system - this helped.

himanshumb
09-22-2008, 05:37 AM
Dear Mr. Jmtbrahma,
For removal of biofilm you sanitize your water Distribution with Hydrogen peroxide , Ozone and peracetic acid . this composition will oxisidize bacteria and biofilm by forming reactive peroxide and free radicals. Hope this will solve your biofilm problem.


Regrds
Himanshu C

morphius909
09-28-2008, 02:16 AM
Biofilm is everywhere...always present to some extent and another..and that is why a good preventative maintenance program is essential.

By the sounds of it, you are looking for a 1x fix?

Unfortunately, this is not going to happen.

Whatever you chose, you must put it on a regular program, 3 months, 6 months, etc, whatever you choose. Up to you, as long as it continues to solve your problem or prevent it.

Minncare is a possibility as well. All the other options are good as well/

pava_satya
10-10-2008, 06:56 AM
Dear Friend,

here two terms one is passivassion and sanitation

Passivation

Typical passivation process of cleaning stainless steel tanks involves cleaning with NaOH and citric acid followed by nitric acid (up to 20% at 120F) and a complete water rinse. This process will restore the film, remove metal particles, dirt, and welding generated compounds (e.g. oxides).

In the context of corrosion, passivation is the spontaneous formation of a hard non-reactive surface film that inhibits further corrosion. This layer is usually an oxide or nitride that is a few atoms thick

Sanitation


sanitation means to reduce the microbial growth by using steam or hydrogen paraxide.

and normally you should avaoid the ded legs and slope calculation s for the distribution line to avaid the water stagnent
and you should check the redigraphy /X rey test for the welding spots to identify the pinholes for the system

everting is ok then frequently you should sanitize the distribution system by passing pure steam for 2 hours is prefferable.


Thanks

Satya
Manager-Quality Assurance
pava_satya@rediffmail.com

XUAN TRAN
10-24-2008, 02:49 AM
We often practice the following procedure :
1. To remove biofilm, by soaking the RO membrane, 30min, with solution 1, pH 10 : either Sodium polyphosphate 2% + Sodium EDTA 0.8%
or Sodium polyphosphate 2% + Sodium Dodecylbenzen sulfonate (DDBS) 0.25%, for thick biofilm.

2. To remove mineral residues, by soaking RO membrane with solution 2% citric acid, 30min.

3. Rinsing the RO system with RO water until obtaining the specified conductivity.

meyert
10-24-2008, 02:35 PM
How do you know you have bio film?

markpelzers
11-04-2008, 12:39 PM
@Meyert: Every ambient watersystem without a periodic sanitization tool will have biofilm. Do not rely on the microbiological results only. The chance that during sampling you catch a part of your biofilm is not that great. If you have a trend of microbiological out of specs, then you are probably too late and your biofilm is not in control. The only solution then is sanitization.


@jmtbrahma:
When you want a good answer on this question, you must supply more information.
Is the watersystem an ambient system? Is it the clean steam system, water for injection system, purified water system? Is your piping plastic or stainless steel?

Normally, the best way of removing biofilm in a water system is heat sanitization or ozon sanitization. But not all methodes can be used in your system.

The problem with biofilm is the protective layer of the biofilm.
This layer excists mainly from protein and protects the colonies against chemical substances, heat, etc.
Ozon will oxidizes this layer and then you can destroy the colonies.
Heat (>85°C) will denaturize the portein structure and has the same result as ozon.

Whenever you have a microbiological out of spec, you don not want to passive every time.....
After passiviation you still needs to sanitize your system.

mickawa
01-26-2011, 01:03 PM
@Meyert: Every ambient watersystem without a periodic sanitization tool will have biofilm. Do not rely on the microbiological results only. The chance that during sampling you catch a part of your biofilm is not that great. If you have a trend of microbiological out of specs, then you are probably too late and your biofilm is not in control. The only solution then is sanitization.


@jmtbrahma:
When you want a good answer on this question, you must supply more information.
Is the watersystem an ambient system? Is it the clean steam system, water for injection system, purified water system? Is your piping plastic or stainless steel?

Normally, the best way of removing biofilm in a water system is heat sanitization or ozon sanitization. But not all methodes can be used in your system.

The problem with biofilm is the protective layer of the biofilm.
This layer excists mainly from protein and protects the colonies against chemical substances, heat, etc.
Ozon will oxidizes this layer and then you can destroy the colonies.
Heat (>85°C) will denaturize the portein structure and has the same result as ozon.

Whenever you have a microbiological out of spec, you don not want to passive every time.....
After passiviation you still needs to sanitize your system.

As I know, after some time the biofilm becames growing. We have a monitoring program for every PW system so can compare the results of bioburden for each system. Now, the periodical sanitisation will prevent growth of bacteria in the water, but will not destroy the biofilm. Older system needs sanitisation more frequently than the newer one. As I spoke with our lab the passivation (using acid) could destroy the biofilm. I did not check it yet, maybe with some changes of systems I will need to make the passivation and then will check results.

Regards.

DURGA PRASAD
01-26-2011, 01:55 PM
Passivation need not be done with HN03. Biotech industry is moving towards alternatives.Recently we have found in a Biotech industry the experts there tried with Citric acid. It is not harsh and it can effectively remove Biofilms. They used a concentration of 0.5M.
They washed whole system with 4 volumes of Distilled water to flush out remaining Citric acid and this was found out by PH and conductivity mesaurements.
Regards

DURGA PRASAD
02-28-2011, 02:19 PM
Of all the methods its cheapest way to sanitize the water systems by Ozone method. In reality its not at all necessary to sanitize the water system when the Bioburden or Conductivity or Chemical charecterestics of water comply with in the pharmacopial or compendial guidelines.
Its a polocy by QA and QC insist always to sanitize the system weekly once.
Its also good to sanitize the whole system with 85C water , but its one of the most easy methods and expensive methods too.

Regards